13th Biotechnology Congress
San Francisco, USA
Jamia Hamdard, India
Title: Deep sequencing of root endophyte Piriformospora indica grown under salt stress
Biography: Nivedita Lal
Background: Piriformospora indica , a filamentous fungus of the order Sebacinales, is able to make symbiotic interaction with root of different plant species and provides better growth and higher yield to the host plant as well as resistance against biotic and abiotic stresses. High soil salinity, excess of NaCl, is one of the important environmental factors that limits distribution and productivity of major crops. The need to produce crops with enhanced tolerance to salt stress has been the stimulus for research. P. indica-mediated salt tolerance mechanism was found to be linked strongly with increase in antioxidants under salt stress in barley which attenuates the NaCl-induced lipid peroxidation, metabolic heat efflux, and fatty acid desaturation in barley leaves. Salt stress studies have indicated promising effect of P. indica in barley. Therefore, it is vital to isolate and functionally characterize salinity stress-related genes to elucidate the mechanisms underlying halotolerance and develop salinity stress-tolerant plants.
Observations: We have compared the transcriptome of P. indica growing under high salt conditions (0.5 M NaCl) with salt free conditions as a control. Approximately 30‐40 million 76bp paired-end reads per sample were obtained using an Illumina NextSeq500. RNA-seq analysis was performed using Bowtie/TopHat/Cufflinks software pipeline. Total 15410 unigenes were generated with n50 value of 3038. A total of 13461 differentially expressed genes (fold change ≧ 2) were identified and 2646 genes were downregulated while 2446 genes were upregulated under high salt condition. We found that the genes involved in different cellular processes,such as metabolism, energy and biosynthetic processes, DNA repair, regulation of protein turnover, transport and saltstress tolerance were changed under high salt condition.
Conclusions: RNA-seq and pathway analyses found that salt stressed P. indica have significant differences in gene expression. Our results showed the complex mechanism of P. indica adaption to salt stress and it was a systematic work for endophyte to cope with the high salinity environmental problems. Thus, these results could be helpful for further investigation of the salt resistance mechanism in microbes.