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13th Biotechnology Congress

San Francisco, USA

Inkyu Park

Korea Institute of Oriental Medicine, South Korea

Title: Comparative analysis of complete chloroplast genome sequence of two Aconitum species and in the family Ranunculaceae

Biography

Biography: Inkyu Park

Abstract

Aconitum species are well known herbaceous medicinal ingredient as well as toxic material and has great economic value in Asian countries. However, genomic information is still limited in Ranunculaceae. In this study, we completed chloroplast genome sequence of two Aconitum species, A. coreanum and A. carmichaelii, based on the Illumina miseq platform. The gene order, gene content and orientation of two Aconitum cp genomes exhibit the general structure of flowering plants, and are similar to other Aconitum species. The two Aconitum chloroplast genomes are 155,880 and 157,040 bp in length, respectively, and contain 131 unique functional genes including  86 protein coding gene, 8 rRNA and 37 tRNA. We established genetic relationship of Aconitum species and Ranunculaceae through phylogenetic tree based on 71 protein condign genes of 19 angiosperms. Comparison of the cp genome structure and gene order to those of Aconitum species revealed general contraction and expansion of the inverted repeat region (IR) and single copy boundary regions. We obtained barcoding target sequence and developed SCAR marker helpful for discrimination of the Aconitum species. These results suggest that the sequence variables of chloroplast genome could provide the useful genetic information and development of molecular marker for discrimination to identify Aconitum species