Meet Inspiring Speakers and Experts at our 3000+ Global Conference Series LLC LTD Events with over 1000+ Conferences, 1000+ Symposiums
and 1000+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business.

Explore and learn more about Conference Series LLC LTD : World’s leading Event Organizer

Back

6th World Congress on Biotechnology

New Delhi, India

Gaurav Baranwal

Gaurav Baranwal

Amrita institute of Medical Sciences, India

Title: O-acetylation of peptidoglycan affects ex-vivo and in vivo survival of S. aureus

Biography

Biography: Gaurav Baranwal

Abstract

Lysozyme is one of the principle components of the host innate defense system which cleaves the β-1, 4 glycosidic bonds between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) of the peptidoglycan to induce bacterial lysis. It is present in a very high concentration in all human biological fluids such as saliva, nasal secretions, serum and tears and produced by neutrophils, macrophages and dendritic cells during phagocytic killing of bacterial pathogen as a part of host innate immune defense mechanism. Staphylococcus aureus (S. aureus), an opportunistic pathogen acetylates its peptidoglycan at the C-6 position of NAM producing the 2, 6-N,O-diacetylmuramic acid derivative to resist the catalytic activity of lysozyme. Earlier studies showed that under in vitro conditions S. aureus mutant with de-O-acetylated peptidoglycan (∆oatA) was 2-3 fold more sensitive towards lysozyme than the parental strain. In the present study, we are reporting the role of peptidoglycan O-acetylation in S. aureus virulence in ex vivo and in vivo experiments. Our preliminary results showed the diminished survival of the mutant devoid of O-acetylation in phosphate buffer solution containing human lysozyme. The survivability of the ∆oatA mutant was also challenged when exposed to human biological fluid like tears, blood, sweat and saliva. Further the survivability inside macrophages as well as under in vivo scenario of the ∆oatA mutant when compared with wild type strain of S. aureus was found diminished. With the above found results we assume OatA could act as a potential target for future drug development.