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6th World Congress on Biotechnology

New Delhi, India

Lokesh Gautam

Lokesh Gautam

Department of Animal Genetics and Breeding , India

Title: Microsatellite Markers Studies For Genetic Characterisation Of Bikaneri Camel

Biography

Biography: Lokesh Gautam

Abstract

Genetic variation in Bikaneri Camel was analysed using eleven microsatellite primers. A total of eleven microsatellite primers were tested for amplification on apanel of 30 unrelated camels. Polymerase Chain reaction was employed for amplification of eleven microsatellite loci.The PCR amplified products were separated by denaturing gel electrophoresis. The gel was stained with silver nitrate procedure.The allele frequency ,expected heterozygosity and polymorphic information content were observed. Six out of eleven (54%) microsatellite primer pairs gave a polymorphic band. The number of alleles ranged from two to five in Bikaneri Camel for different microsatellite markers. The expected heterozygosity ranged from 0.289( VOLP -08) to( VOLP-10) and the polymorphic information content values in camel ranged from 0.267 (VOLP-) to 0.639 (VOLP-10).On the basis of all over observation,it was revealed that microsatellite primers were found to be polymorphic with good polymorphic information content . Hence they can be used for further genetic studies.