6th World Congress on Biotechnology
New Delhi, India
Manav Rachna International University, India
Title: Identification and Characterization of Bacteriocins isolated from soil bacteria and comparison between two characterized bacteriocins.
Biography: K.L.R. Bonhi
Bacteriocins are an abundant and diverse group of ribosomally synthesized antimicrobial peptides produced by bacteria inhibit the growth of similar or closely related bacterial strain. The can be used as alternative to antibiotics. The aim of our research work was to identify and characterized the bacteriocins produced by soil-associated bacteria. subtilin from Bacillus subtilis Bs and pyocin from Pseudomonas aeruginosa Pa were found active against gram-positive microorganism tested. Production of both the bacteriocins started and observed during the stationary phase. Maximum production of both the bacteriocins was observed at 30 degrees C in BHI medium. Subtilin Bb remained stable during 4-8 pH while pyocin Pa remained stable at pH 1-11. Activity of both was completely lost after proteinase K treatment suggesting their protein nature. Both of the bacterocins were found resistant to high temperature (121 degrees C for 30 min), detergents (1% solutions of EDTA, Tween 20 and SDS) and solvents (1% solutions of Ethanol, Methanol,Chloroform, and Acetone) Titre of Subtilin Bb was estimated to be 4680 AU/mL while the titre for pyocin Pa was calculated as 780 AU/mL. Both of the bacteriocins showed bacteriolytic mode of action against the indicator Bacillus strain BC31 and were found <25 KDa in their molecular mass.