Meet Inspiring Speakers and Experts at our 3000+ Global Conference Series LLC LTD Events with over 1000+ Conferences, 1000+ Symposiums
and 1000+ Workshops on Medical, Pharma, Engineering, Science, Technology and Business.

Explore and learn more about Conference Series LLC LTD : World’s leading Event Organizer


6th World Congress on Biotechnology

New Delhi, India

Kalyani Khanra

Panskura Banamali College, India

Title: HeLa cells containing a truncated form of DNA polymerase beta are more sensitized to alkylating agents than to agents inducing Oxidative stress


Biography: Kalyani Khanra


The present study aimed at determining the effect of alkylating and oxidative stress inducing agents on a newly identified variant of DNA polymerase beta (polβ208-304) specific for ovarian cancer. Polβ208-304 has deletion of exons 11-13 which lies in catalytic part of enzyme. We compared the effect of these chemicals on HeLa cells and HeLa cells stably transfected with this variant cloned into in pcDNAI/neo vector by MTT assay, colony forming assay and apoptosis. Polβ208-304 cells exhibit greater sensitivity to alkylating agent and less sensitivity towards H2O2 and UV when compared with HeLa cells alone. It has also been shown that cell death in Polβ208-304 transfected HeLa cells is mediated by the caspase-9 cascade. The exon-11 have nucleotidyl selection activity, exon-12 and 13 have dNTPs selection activity. Hence deletion of this part may affect polymerizing activity although single strand binding and double strand binding activity may remain same. Missing of this part may adversely affect catalytic activity of DNA polymerase beta hence this variant may act as a dominant negative mutant. This would represent a clinical significance if translated into clinical setting because resistance to radiation or chemo during the relapse of the disease could be potentially overcome by this approach.