6th World Congress on Biotechnology
New Delhi, India
Division of Fruits and Horticultural Technology, ICAR-IARI, India
Title: Characterization of Guava Genotypes using RAPD Markers
Genetic diversity among 24 genotypes (22 varieties / collection belonging to Psidium guajava and 2 other species) of guava were characterized using Random Amplified Polymorphic DNA (RAPD) markers. Out of 29 RAPD primers used, 10 were found to be monomorphic and 19 showed polymorphism among guava genotypes. Number of alleles detected using polymorphic RAPD primer ranged between 2 (OPA13A) to 11 (OPF02A) with an average of 6 amplicons / primer. High rate of polymorphism was observed reasonably for OPF02A, OPH19A, OPF13A, OPA13A and OPB13A primers. The PIC value ranged from 0.49 to 0.89, indicates that the markers were quite informative. Based on molecular anlaysis, Sasri Selection and Sasni Selection were grouped with Allahabad Safeda. Tamil Nadu Selection and Lalit genotypes formed a group at 50% similarity. Psidum freidrichstahlianum formed a group with Black guava. Molecular analysis showed a high degree of variation among analyzed guava genotypes indicating an important source of genetic diversity that can be used in the guava improvement programme.