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6th World Congress on Biotechnology

New Delhi, India

Jyoti Kushwah

Jyoti Kushwah

Indian Agricultural Research Institute, New Delhi

Title: Analysis of factors governing nematode-host specificity in bacterium Photorhabdus

Biography

Biography: Jyoti Kushwah

Abstract

Majority of animals form symbiotic relationships with bacteria. Some animals partner with a single bacterial symbiont, while other animals symbiose with simple, or complex bacterial consortia. The insect parasitic nematode Heterorhabditis lives in mono-specific symbiotic relationship with the enterobacteriumPhotorhabdus and is a tractable genetic model for the study of animal-microbe relationships. Heterorhabditis - Photorhabdus association is highly species-specific. Present work was undertakento identify factors which might be involved in the host specificity of the bacterial symbionts Photorhabdus with its nematode partner Heterorhabditis. Total protein profile of 2Photorhabdussubspecies, P.luminescens subsp laumondii (isolated fromH. bacteriophora) and P. luminescens subspakhurstii(isolated fromH. Indica) was compared by 2D Gel electrophoresis followed by identification of differentially expressed proteins by MALDI-TOF MS.Out of43 proteins analyzed, 13unique proteins were identified in P.luminescens subsp laumondii, comprising2 catalases, 3 ABC-type transporters, 1 translation elongation factor, 1 outer membrane Protein, 1 peptidase,1 chaperon protein, 2 antibiotic synthesis regulators and 2 hypothetical proteins. Remaining 20 were from P. luminescens subspakhurstii, showing 2 transcription regulators, 4 dehydrogenases, 2 ABC-type transporter, 1 DNA topoisomerase, 1 catalase, 1 kinase, 2 Tol B (proteinimport), 2 involved in amino acid synthesis, 2 involved in phosphatebiosynthesis and 3 hypothetical proteins. Here we identified some of the proteinsthat might be involved in nematode-host specificitybetween Heterorhabditis and Photorhabdus.