17th Euro Biotechnology Congress
Patho-Logica, Scientific Park Ness Ziona, Israel
Title: Free floating brain sections for immunofluorescence markers: A technical and scientific approach
Biography: Emmanuel Loeb
Free floating sections is regarded as a new histological method that can be used for immune fluorescence staining. This method is clearly the best way to go for optimal Ab expression in the tissue. Furthermore, staining of thick sections can later on be used for a confocal microscopical analysis. This presentation covers the technical work pattern of the method starting with the tissue preparation and conservation, threw brain accurate dissection and staining. The method is very suitable for morphometry quantification of histological data, here method of image analysis will be presented and the scientific value will be discussed. Furthermore, examples are presented of projects that had combined the method such as stroke and Parkinson models in lab animals. Finally a discussion will be presented were the advantages of the current method will be pointed compared to the classical immunohistochemistry methods.