17th Euro Biotechnology Congress
Centro Universitário da FEI, Brazil
Title: Application of laccase during the attainment of PVA hydrogels reticulated with ferulic acid
Biography: Andreia de Araújo Morandim-Giannetti
Many researches have been aiming at obtaining new possible vitreous humor substitutes. Therefore, we performed studies related to obtain hydrogels enzymatically cross-linked with ferulic acid. The hydrogels were obtained by varying PVA mass, enzyme concentration and the mass of ferulic acid, to obtain the best condition to have a material with similar characteristics in terms of density (1.0053 to 1.0089 g/mL), kinematic viscosity (greater than 4mm2/cm) and refractive index (between 1.3345 and 1.3348). The data was analysed using the Statistica 12.0 software, and it was possible to determine the best condition for obtaining the material: mPVA=12.05% (m/m), laccase concentration=836 (μg/mL) and ferulic acid concentration=1.95 (mM). Hydrogels were obtained under the best condition and on analyzing by DSC, confirmed the presence of crosslinks in the hydrogels and reinforced the hypothesis of the presence of crosslinks due to the action of the Laccase enzyme. This was justified due to a reduction of the glass transition temperature (69.99 and 74.49 ºC), melt temperature (216.48 and 220.26 ºC) and crystallization temperature (181.82 and 184.62 ºC), as well as the degree of crystallinity (29.18 and 29.74 %) for the hydrogel obtained with and without PVA, ferulic acid and laccase, respectively. In this case, it is possibly attributed to the greater intensity of the hydrogen bonds between the PVA chains, which makes it difficult to move and pack the chains into crystallites.