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12th EuroBiotechnology Congress

Alicante, Spain

R Sowmya

R Sowmya

Mysore University, India

Title: Influence of AM fungi and Trichoderma viride on growth and active principles in micropropagated Bacopa monnieri (L.) Pennell, a medicinal plant

Biography

Biography: R Sowmya

Abstract

Bacopa monnieri (L.) Pennell is commonly known as “Brahmi” belongs to the family Scrophulariaceae. It is one of the most important medicinal plants, which has fascinated the fields of traditional and modern system of medicine as memory enhancer and nervine tonic. To understand the influence of AM fungi alone and with a phosphate solubilizing organism on micropropagated plant in comparison with normal plants, an attempt has been made in the present study. Micropropagated plants were raised on MS medium supplemented with Kin and BAP from leaf explants. Both normal and micropropagated plants were inoculated with Glomus mosseae and G. fasciculatum alone and in combination with Trichoderma viride in pots. Percent colonization and spore count were recorded in both normal and micropropagated treated and control plants. Micropropagated plants showed early and better colonization with AMF association compared to normal plants. Plant growth and biomass were recorded for treated and control of normal and micropropagated plants. AMF enhanced the growth of micropropagated plants considerably with G. mosseae in combination with T. viride compared to control plants. Micropropagated inoculated plants showed increased level of chlorophylls, total proteins and total phenolics but reducing sugars and total carbohydrates content showed decreased level compared to control plants. The increased level in the Bacoside A-content was recorded in plants treated with G. mosseae along with T. viride. The micropropagated B. monnieri plants responded excellently to the AMF inoculation with T. viride and showed better establishment in the pots by availing good growth and improved physiological condition.