12th EuroBiotechnology Congress
Georgian Technical University, Georgia
Title: Hormonal treatment effect in combination with sucrose on in vitro potato varieties
Biography: Maia Kukhaleishvili
In vitro plant reproduction widely used in agriculture and biotechnology and has a great advantage compared to traditional breeding methods allows to obtain get planting material in a short-term and plant reproduction can be conducted throughout the year. The present investigation was carried out to select best MS medium protocol and identify perfect hormonal/sucrose combination for three varieties of potato (Solanum tuberosum L): “Nevski”, “Riviera” and “Zefira” for their in vitro cultivation. Potato varieties were studied on three types of MS medium: MS+30 g/l sucrose (6% MS medium), 6% MS medium +1 mg/l BAP+0.05 mg/l IBA and 6% MS medium+1 mg/l BAP+0.1 mg/l IBA. It was revealed that high concentration of IBA has negative effect on plants development, respectively 6% MS medium+1 mg/l BA+0.1 mg/l IBA was not optimal neither cultivars of potato. According to the results cultivars Zefira and Riviera had maximum potential for in vitro rooting (correspondingly: 82.97% and 100%) and shoots (100% and 87.34%) formation on 6% MS medium+1 mg/l BA+0.05 mg/l IBA but Nevsky gave maximum development (rooting 82% and shoot formation 87%) on 6% MS medium. In conclusion, both hormones combination presented in experiment with 30 g/l sucrose showed optimal result on in vitro growing potato cultivars Zefira and Riviera but best in vitro cultivation of Nevsky was revealed MS medium supplemented only 30 g/l sucrose without growth hormones, probably Nevsky has ability to produce itself the amount of hormones which is necessary for its normal growing.