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8th Euro Biotechnology Congress

Frankfurt, Germany

S. K. Tiwari

S. K. Tiwari

State Forest Research Institute, Jabalpur India

Title: Standardization of clonal macropropagation protocol of Dillenia pentagyna roxb an important and endangered medicinal tree species through stem branch cuttings

Biography

Biography: S. K. Tiwari

Abstract

Abstract: Dillenia pentagyna belongs to family Dilleniaceae , is commonly known as Karkat. It is small deciduous tree reaching up to the height of 10-12mt with a straight bole. The species is distributed throughout in India including Andaman & Nicobar. It is highly important medicinal tree species. Its leaves, fruits and bark shows antibacterial, anti-alpha glucosidase and antioxidant property. Due to its high medicinal value the natural occurrence of this species is declining day by day and gradually the species comes under the threat condition. The species is highly recalcitrant in nature, because it very difficult to propagate by conventional propagation methods. Now there is an urgent need to develop an appropriate technology for its conservation and clonal propagation. An attempt has been made for standardizing the clonal propagation technique of this valuable medicinal species through stem branch cuttings under intermittent misting conditions in mist chamber. The optimum rooting response has been standardized by various PPM concentrations and treatment timings of root promoting hormones IBA and NAA. The optimum rooting response > 60 percent was observed when the cutting were treated up to 20 minute with 500 ppm concentration of IBA. On an average 6 roots with10-15cm length were induced from the cuttings after 30-35 days.NAA was failed to induce roots from the cuttings