Yung-Chih Kuo
National Chung Cheng University Taiwan
Title: Rescuing apoptotic neurons from Alzheimer’s disease using wheat germ agglutinin-conjugated and cardiolipin-conjugated liposomes with encapsulated nerve growth factor and curcumin
Biography
Biography: Yung-Chih Kuo
Abstract
Liposomes with cardiolipin (CL) and wheat germ agglutinin (WGA) were developed to permeate the blood–brain barrier (BBB) and treat Alzheimer’s disease. WGA-conjugated and CL-incorporated liposomes (WGA-CL-liposomes) were employed to transport nerve growth factor (NGF) and curcumin (CUR) across a monolayer of human brain-microvascular endothelial cells regulated by human astrocytes and to protect SK-N-MC cells against apoptosis induced with β-amyloid1-42 (Aβ1-42) fibrils. An increase in the CL mole percentage in lipids increased the liposomal diameter, absolute value of zeta potential, entrapment efficiency of NGF and CUR, release of NGF, biocompatibility, and viability of SK-N-MC cells with Aβ1-42, however, decreased the atomic ratio of nitrogen to phosphorus and release of CUR. In addition, an increase in the WGA concentration for grafting enhanced the liposomal diameter, atomic ratio of nitrogen to phosphorus, and permeability for NGF and CUR across the BBB, however, reduced the absolute value of zeta potential and biocompatibility. WGA-CL-liposomes carrying NGF and CUR can be promising colloidal delivery carriers to target the BBB and inhibit neurotoxicity for future clinical application.
