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8th Euro Biotechnology Congress

Frankfurt, Germany

Hattem M Mekky

Hattem M Mekky

University of Alexandria Egypt

Title: Biological activity of elicited Echinacea purpurea suspension cultures

Biography

Biography: Hattem M Mekky

Abstract

Chitosan, phenyl alanine (Phe) and methyl jasmonate (MeJA) significantly increased the production of polyphenolics in suspension cultures of E. purpurea initiated on MS supplemented with 1.5 mg/L BA and 0.5 mg/L NAA. However, cultures elicited with 4 mM copper sulphate (CuSO4) possessed the highest EC100 in cytotoxicity experiments. Furthermore, Phe, MeJA, CuSO4 and chitosan significantly increased ABTS scavenging properties. Additionally, MeJA and Phe extracts stimulates the phagocytic and yeast intracellular killing activities of PMN and Finally, the anti-inflammatory properties of MeJA elicited cultures were the highest and were displayed by its strongest nitric oxide scavenging activity with an EC50 6.3 µg/ml and the most powerful inhibition of lymph proliferative activity induced by lipopolysaccharides with an EC50 4.5 µg/ml.