Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 21st European Biotechnology Congress Holiday Inn Vinogradovo, Moscow, Russia.

Day 2 :

Keynote Forum

Igor l Katkov

Belgorod National State Research University, Russian Federation

Keynote: Stopping biological time: Science and art of biostabilization

Time : 10:00-10:40

Conference Series Euro Biotechnology 2018 International Conference Keynote Speaker Igor l Katkov photo
Biography:

Igor L Katkov is a trained biophysicist with 30+ years of experience in cryobiology and cryogenic engineering. His last years of research have been focused on the fundamental aspects of kinetic vitrification (K-VF) as well on designing the practical system for K-VF KrioBlast™ (in cooperation with V F Bolyukh). Currently, the Head of the Laboratory of the Amorphous state at the Belgorod National Research University BelSU, Russia. He has recently accepted a
Professor level position as the Head of the Laboratory of Cryobiology at the V I Kulakov Research Center of Obstetrics, Gynecology and Perinatology (RCGOP), Moscow, Russia and Chief Scientific Officer of Celltronix, San Diego, CA, USA.

Abstract:

Biostabilization (a.k.a. biopreservation) is a process that leads to cessation of the basic chemical and biological reactions
so the biosamples can be pooled and stored (biobanked) for long time. There are 5 basics ways of achieving long-term
storage, which ALL essentially lead to vitrification of cells, namely: slow freezing (SF), equilibrium vitrification (E-VF), kinetic
vitrification (K-VF), freeze-drying (lyophilization) and vacuum/air flow drying at temperatures above 0°C (xeropreservation).
Different combinations of the 5 basic biopreservation technologies such a preliminary drying before cryogenic slow freezing
or vitrification is also possible. Author will discuss a phase diagram that shows all 5 basic ways of biostabilization and will
discuss pros and cons of all approaches. A special emphasis will be put on the kinetic vitrification as it does not require the
high concentrations of (or does not need at all) potentially toxic and osmotically damaging exogenous permeable intracellular
vitrificants (also called cryoprotectants). Author will also present KrioBlast-2, a pilot version of the KrioBlast™ platform for
cryopreservation by K-VF. Preliminary experiments on K-VF of human pluripotent stem cells and spermatozoa, which
showed an equally excellent (80-90% of the untreated control) will be also discussed. A more advanced version KrioBlast-3
will be discussed in the concurrent presentation.

Keynote Forum

Sergey Suchkov

I M Sechenov First Moscow State Medical University, Russia

Keynote: Antibodies with functionality as a new generation of translational tools to monitor, to predict and to prevent demyelination

Time : 10:00-10:40

Conference Series Euro Biotechnology 2018 International Conference Keynote Speaker Sergey Suchkov photo
Biography:

Sergey Suchkov graduated from Astrakhan State Medical University and was awarded with MD and maintained his PhD and Doctor’s degree. He was working for Helmholtz Eye Research Institute and Moscow Regional Clinical Research Institute. He was a Secretary-in-Chief of the Editorial Board, Biomedical Science, an international journal published jointly by the USSR Academy of Sciences and the Royal Society of Chemistry, UK. Currently, he is a Director of Center for Personalized Medicine, Sechenov University; Chair of the Department for Translational Medicine, Moscow Engineering Physics University and Secretary General of United Cultural Convention, Cambridge, UK. He is a Member of the New York Academy of Sciences; American Chemical Society; American Heart Association; AMEE, Dundee, UK; EPMA, Brussels, EU; PMC, Washington, DC, USA and ISPM, Tokyo, Japan.

Abstract:

Abs against myelin basic protein/MBP endowing with proteolytic activity (Ab-proteases with functionality) is of great value to monitor demyelination to illustrate the evolution of multiple sclerosis (MS). Anti-MBP auto-Abs from MS patients and mice with EAE exhibited specific proteolytic cleavage of MBP which, in turn, markedly differed between: MS patients and healthy controls; different clinical MS courses and; EDSS scales of demyelination to correlate with the disability of MS patients to predict the transformation prior to changes of the clinical course. Ab-mediated proteolysis of MBP was shown to be sequence-specific whilst demonstrating five sites of preferential proteolysis to be located within the immunodominant regions of MBP and to fall inside into 5 sequences fixed. Some of the latter (with the highest encephalitogenic properties) were proved to act as a specific inducer of EAE and to be attacked by the MBP-targeted Ab-proteases in MS patients with the most severe (progradient) clinical courses. The other ones whilst being less immunogenic happened to be EAE inducers very rare but were shown to be attacked by Ab-proteases in MS patients with moderate (remission-type) clinical courses. The activity of Ab-proteases was first registered at the subclinical stages 1-2 years prior to the clinical illness. About 24% of the direct MS-related relatives were seropositive for low-active Ab-proteases from which 22% of the seropositive relatives established were being monitored for 2 years whilst demonstrating a stable growth of the Ab-associated proteolytic activity. Moreover, some of the low-active Ab-proteases in persons at MS-related risks (at subclinical stages of MS), and primary clinical and MRT manifestations observed were coincided with the activity to have its mid-level reached. Registration in the evolution of highly immunogenic Ab-proteases would illustrate either risks of transformation of subclinical stages into clinical ones, or risks of exacerbations to develop. The activity of Ab-proteases in combination with the sequence-specificity would confirm a high subclinical and predictive (translational) value of the tools as applicable for personalized monitoring protocols. Ab-proteases can be programmed and re-programmed to suit the needs of the body metabolism or could be designed for the development of principally new catalysts with no natural counterparts. Further studies on targeted Abmediated proteolysis may provide a translational tool for predicting demyelination and thus the disability of the MS patients.

Keynote Forum

Igor l Katkov

Belgorod National State Research University, Russian Federation

Keynote: KrioBlastTM-3 - a three module system for efficient cryopreservation of unfreezable cells
Conference Series Euro Biotechnology 2018 International Conference Keynote Speaker Igor l Katkov photo
Biography:

Igor L Katkov is a trained biophysicist with 30+ years of experience in cryobiology and cryogenic engineering. His last years of research have been focused on the fundamental aspects of kinetic vitrification (K-VF) as well on designing the practical system for K-VF KrioBlast™ (in cooperation with V F Bolyukh). Currently, the Head of the Laboratory of the Amorphous state at the Belgorod National Research University BelSU, Russia. He has recently accepted a Professor level position as the Head of the Laboratory of Cryobiology at the V I Kulakov Research Center of Obstetrics, Gynecology and Perinatology (RCGOP), Moscow, Russia and Chief Scientific Officer of Celltronix, San Diego, CA, USA.

Abstract:

As we have stated before, there are 5 basics ways of achieving long-term storage, which ALL essentially lead to vitrification of cells, namely: slow freezing (SF), equilibrium vitrification (E-VF), kinetic vitrification (K-VF), freeze-drying (lyophilization), and va San Diego vacuum/air flow drying at temperatures above 0oC (xeropreservation). Previously, we presented KrioBlast-2, a pilot version of the KrioBlast™ platform for cryopreservation by kinetic (very fast) vitrification. One of the major advantages of K-VF over the existing approach for vitrification (E-VF) is that K-VF does not need the high concentrations of potentially toxic and intracellular vitrificants (also called: cryoprotectants, which is not exactly correct in this case) such as DMSO, ethylene glycol, dimethyl sulfamide. The pilot experiments on human pluripotent stem cells and spermatozoa, which showed an equally excellent (80-90% of the untreated control), were presented. The other key advantage of K-VF is its universality so the system is equally suitable for any kind of cells and tissues as soon as the characteristic thermal time of the system, which basically depends on the geometry of the cryo container with the sample, is sufficiently short. In this presentation, we will present the future development, the industrial three module system KrioBlast-3 that comprises 1) the cooling chamber for hyperfast cooling, 2) the intermediate module for shipment or long term storage in liquid nitrogen, and 3) the rewarming module. The second module has two port sites for the cooling and the rewarming modules so the system resembles a space station. All operations of cooling, storage/shipment, and warming are done without any contact of the sample with the ambient environment. The specific cryo containers for K-VF, namely VitriPlateTM, VitriCombTM, and VitriScanTM for vitrification of cells in suspension, packed in straws, and attached to surface in multiwell systems respectively are also discussed.

  • Oncolytic Biotechnology| Molecular Biotechnology and Genetics| Microbial Biotechnology
Location: Winter garden
Speaker

Chair

Sergey Suchkov

I M Moscow State Medical University, Russia

Session Introduction

Mihai Gidea

University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania

Title: Research on the testing of products with biostimulatory effect based on amino acid with potential in the treatment of rape seed
Speaker
Biography:

Mihai Gidea is form University of Agronomic Sciences and Veterinary Medicine of Bucharest, Romania

Abstract:

Due to its major potential for biofuel production, the rape-cured areas have steadily increased lately, reaching a cultivated area of 38 million ha worldwide at 2016 (FAOSTAT). In these conditions, the problem of increasing the
level of the obtained productions is raised more and more, The paper presents the results of the researches regarding the treatment of rape seeds with products containing amino acids (aa). In this context, there were 4 products based on keratin hydrolyzed wool and chelated (co) of Zn, Mn, Cu, Mg and Mo. For testing, a bifactorial experience was performed where Factor A tested the product with 5 graduations a1 14% aa + 0.5% co, a2 12% aa + 0.3% co, a3 10% aa + 0.4% co, a4 14% aa+0.4% co+1% Caryophyllus aromaticus oil microcapsules, and Factor B seed immersion time in products tested with a1 control, a2 1h, a3 2h, a4 3h. The assays were performed under laboratory conditions. The treatments were film-coated and, after treatment, the seeds were seeded, the TopPaper recommended by ISTA for rapeseed testing.The research found that all the treatments applied had a stimulating effect on the monitored parameters, thus increased the rate and germination rate, and increased the average daily germination rate, the average germination time. From a biometric point of view, there was an increase in the average length of plantlets and roots, as well as the average daily growth rate. The treatments applied did not show phytotoxic effects.

Biography:

Maryam Ranjpour Aghmiouni has completed her PhD from Jamia Hamdard University. She has applied for Post-doctoral position at Illinois University
and her application is under process. She has published two manuscripts and three more manuscripts are under re-revision status at high repute
journals.

Abstract:

Liver cancer is the third common cancer to cause maximum death among patients diagnosed with cancers. The search for new biomarker discovery is necessary as none of the identified biomarkers alone are enough sensitive toward hepatocellular carcinoma (HCC). In order to find out novel biomarkers that can diagnose HCC at very early stage, we have developed a rodent model using chemical carcinogens, N-Nitrosodiethylamine (DEN) and 2 aminoacetylfluorine (2AAF). The disease progression was monitored by histological evaluation. Proteomic approaches such as 2D-Electrophoresis, LCMS/MS and Western blot analyses have been used to analyze the differentially expressed proteins in carcinogen-treated animals vis-a-vis controls. The total serum proteins were isolated, solubilized and resolved on 2D-Gel Electrophoresis using broad pH range IPG strips. PD-Quest analysis revealed proteins that are differentially expressed in serum of the carcinogen-treated rats as compared to controls. Some of these proteins have been identified by LCMS/MS. Histological analysis confirmed liver inflammation and disease initiation at one month and tumorigenesis at four months after carcinogen treatment. One of the differentially expressed proteins, namely, cytosolic phospholipase A2 delta was significantly up-regulated at very early stage of cancer development and continued to remain elevated with disease progression up to tumor stage. The increase in its expression has been confirmed by Western blot analysis. Further, the analysis of serum of liver cancer patients also showed elevated expression of this protein that validated our experimental data. The study suggests that elevation in cytosolic phospholipase A2 delta expression is associated with progression of HCC.

Speaker
Biography:

Abstract:

Objective: The research was aimed at analysing single nucleotide polymorphisms and haplotypes on D-loop and Cyt-b regions of the mitochondrial DNA of tilapia fish.
Methods: Fifteen and thirteen tilapia fish were obtained from two populations, South-South (Domita farm) and South West (Odeda farm). DNA extraction from fish tissue was done using Quick-gDNATM mini prep kit
after which PCR amplification was carried out. Sequencing of the two mtDNA regions were done using forward primer 5’- GGATTYTAACCCYTRCCCC- 3’ and reverse 3’-AGTAAGTCAGGACCAAGCC-5’ for D-loop and
5’-GGATTTTAACCCTTACCCC-3’ and 3’AGTAAAGTCAGGACCAAGCC-5’ for Cyt-b region. Statistical analyses were carried out on the aligned sequenced data using MEGA version 6.06, DnaSP 5.1, Codon code aligner 6.06 as well as NETWORK 4.6.1.1.
Results: mtDNA polymorphism was highest in the D-loop of South-South (SS) population with 176 polymorphic sites, while South-West (SW) population had 162 polymorphic sites translating to 176, 162 and 144 SNPs with nonsynonymous substitutions higher than synonymous substitutions. Haplotype diversities (Hd) were 1.00±0.024 and 1.00±0.030 while nucleotide diversities were 0.168±0.086 and 0.161±0.084 for D-loop of SS and SW populations, respectively. For Cyt b region, haplotype and nucleotide diversities were 0.91±0.003 and 0.051±0.016. Positive selection was more on mtDNA D-loop of tilapia sampled from SS than those from the SW as well as Cyt-b region of tilapia fish from SS. 28 haplotypes were identified among the tilapia from SS and SW with no shared haplotypes while 9 haplotypes were identified from the Cyt-b region with haplotypes 4, 5, 6 and 7 shared between species. Median joining network analysis revealed population-based clustering pattern. Demographic expansion was not significant using Tajima’s D and Fu’s F statistics.

Speaker
Biography:

Ferhat Djoudi has completed his PhD on Epidemiology and molecular characterization of MRSA in 2015 at Abderrahmane Mira University of Bejaia, Algeria. And he started Postdoctoral studies at the same university, on MDR and XDR tuberculosis in Algeria. He is the Head of Microbiology Department and Teacher-Researcher at the same university. He has published many papers in reputed journals.

Abstract:

Tuberculosis is an old infectious disease and the causative agent is Mycobacterium tuberculosis complex. The direct diagnosis stills long and fastidious since bacilloscopy, even if is fast, lacks sensitivity. The culture on Lowenstein- Jensen (L-J), which remains the reference method with a good sensitivity, sometimes takes up to ten weeks to obtain the result. In order to compensate the slow growth of cultures on solid media, new automated methods have been developed, including BACTEC MGIT 960, Versa TREK, MBRedox, BACTEC 460, which allow early diagnosis and more suitable for antibiotic therapy, in addition to their good sensitivity and specificity. The aim of this study is to verify the contribution of BACTEC MGIT 960 in the diagnosis of pulmonary tuberculosis, compared to basciloscopy and classic culture on L-J medium, at the Tuberculosis and Mycobacteria unit in Pasteur Institute of Algeria. Nine hundred and fourteen (914) specimens were collected between January 2016 and April 2017. One hundred and seventy nine (179) cases were reported positive by L-J classical culture and/or BACTEC MGIT 960. Among the 179 cases, 155 were detected by the BACTEC MGIT 960 system, and confirmed by Ziehl control, L-J subculture and MPT64 immuno-chromatographic assay. On classic L-J culture and bacilloscopy, nevertheless, only 123 and 95 specimens respectively were positive. These results confirm the height susceptibility of BACTEC MGIT 960 in improving the diagnosis of tuberculosis in bacilli-poor specimens, compared to classic culture (p=0.037) and direct examination (p=0.014). Furthermore, the contamination rate was higher in L-J culture: 81/914 (8.86%), including 7 bacilloscopy positive specimens, whereas, with BACTEC MGIT 960, only 29/914 (3.17%) specimens were contaminated, with no positive bacilloscopy cases. This result was statistically confirmed (p<0.0001). However, on the 95 bacilloscopy positive specimens, 6 did not give positive cultures neither on BACTEC MGIT 960 nor on L-J. The main advantage of BACTEC MGIT 960 is its ability to shorten the time of mycobacterial growth to an average of 7 days, compared to the solid medium. Nevertheless, the bacilloscopy and culture on L-J remains complementary to this automat, for a reliable diagnosis. Despite the good laboratory practices, there is an incompressible risk of contamination.