Day 1 :
French-German Research Institute of Saint-Louis, France
Keynote: Spray flash evaporation for the continuous production of high performance nanodrugs: New challenges for a new disruptive process
Time : 09:30-10:15
Denis Spitzer received his PhD in Physical Chemistry in 1993 at University Louis Pasteur of Strasbourg. He is the founding and current Director of the NS3E Research Laboratory UMR 3208 ISL/CNRS/UNISTRA. He conducts research in continuous nano-crystallization processes of organic nanomaterials such as model medicaments and energetic materials. He is the inventor of the SFE process. He is the author of more than 150 publications and scientific reports.
NS3E laboratory developed the Spray Flash Evaporation (SFE) for preparing drug nanoparticles at industrial scale. The process was several times patented up to now. The solution is kept in a pressurized tank separated from a vacuum chamber by a hollow cone nozzle, used both to heat and spray the liquid. The instantaneous evaporation of the solvent originates from the combination of the abrupt pressure drop and the high energy stored by the overheated solvent prior to nebulisation. The flash evaporation leads to small crystallites with narrow size distribution. The nanoparticles may be composed of single compounds, mixtures of several substances or co-crystals. In the domain of medicaments, co-crystals are of critical importance as they enhance bioavailability and up-take by the human body of Active Pharmaceutical Ingredients (API). Up to now, most used techniques are of batch nature and are not able to give access in big amounts to nano-sized crystals or co-crystals of therapeutic interest. The SFE permits the continuous manufacturing of nano-sized co-crystals, in large amounts with a kinetic complying with the pharmaceutical industry’s requirements. The efficiency of SFE is shown by the manufacturing of pure nano-medicaments but also of nano-co-crystals such as resveratrol/4-aminobenzamide (1/1), caffeine/oxalic acid (2/1) and caffeine/glutaric acid (1/1), with a mean particle size of between 30 and 100 nm. After showing the possibility to continuously nano-crystallize medicaments, the presentation will focus on different main challenges to further enhance the production capacity and also to understand the SFE process itself. Among different techniques and metrologies used or specially developed such as phase Doppler interferometry (Figure) and AFM-TERS spectroscopies, the presentation will also focus on different crystallization configurations used.
Arkansas State University, USA Infinite Enzymes, LLC, USA
Time : 10:15-11:00
Elizabeth E Hood has 35 years of experience in Biology. She is Distinguished Professor of Agriculture at Arkansas State University and; CEO of two biotechnology start-up companies—Infinite Enzymes, LLC and Infinite Eversole Strategic Crop Services, LLC. Previously, she was an Associate Vice Chancellor for Research and Technology Transfer at ASU; Program Director in Molecular and Cellular Biosciences at the National Science Foundation: Leader in forming one of the world’s foremost transgenic plant research groups at ProdiGene, a plant biotechnology company. She has completed her PhD in Plant Biology at Washington University and MS in Botany awarded by Oklahoma State University
Industrial enzymes are excellent technologies to apply in manufacturing to alleviate environmental pollution. Using plant-based materials allows manufacturing of goods from renewable resources. My laboratory and company are engaged in producing enzymes for industrial applications using the plant seed system. The advantages of the seed system are that the production costs can be quite low because scaling-up just involves planting more acres, the seed can be stored for years, and the enzymes are extremely stable in the seed. We use maize grain to express enzyme genes from fungal and bacterial sources that have specific applications in biofuels, bioproducts and waste water remediation. Genes for these enzymes are partially codon optimized and their expression is driven by either an embryo or an endosperm promoter and targeted to one of three subcellular locations—the cell wall, the endoplasmic reticulum or the vacuole. Expressed enzymes include three cellulases, a phospholipase, a Mn peroxidase, and a laccase. Once the gene is expressed and a high-expressing event chosen, breeding into elite inbred germplasm commences. Applications of the various enzymes will be discussed along with regulatory considerations
BioEnergy Society of Singapore, Singapore. Ngee Ann Polytechnic, Singapore
Anli Geng is currently the Assistant Director of Life Sciences and Chemical Technology of Ngee Ann Polytechnic. She currently holds the President position at BioEnergy Society of Singapore (BESS) and she is also the Co-founder and Director of Sunvisiae Biotech Pte Ltd, a Singapore-based industrial biotechnology company. Prior to joining Ngee Ann Polytechnic, she was working at Institute of Environmental Science and Engineering (IESE) as a Research Scientist. She has more than 25 years of R&D experience, working extensively on environmental biotechnology, green energy technology and industrial biotechnology. She has more than 30 journal publications and her work has been presented in many international conferences. Her current research focus at Ngee Ann Polytechnic is developing novel microorganisms to produce industrial enzymes, chemicals and fuels, novel nutraceuticals and cosmetics ingredients. She obtained Ngee Ann Polytechnic Staff Excellence Award and IChemE Award on Sustainable Technology in 2012.
Industrial biotechnology is a set of practices that use living cells (such as bacteria, yeast, algae) or component of cells like enzymes, to generate industrial products and processes. This presentation focuses on the application of industrial biotechnology in technology development for fuel and chemical production. The talk will cover industrial biotechnology application in the development of cellulolytic enzymes and metabolic engineering of yeast for the conversion of lignocellulosic biomass to fuels and chemicals. The conversion of oil palm empty fruit bunch, the main biomass resource in Southeast Asia, will be particularly discussed.