Day 3 :
Tbilisi State Medical University, Georgia
Keynote: Biomacromolecule from medicinal plants, its synthetic basic monomeric moiety and their anticancer activity
Time : 10:00-10:50
Vakhtang Barbakadze in 1978 and 1999 has completed his PhD and Doctor of Science at the ages of 33 and 54 years from Zelinsky Instiute of Organic Chemistry, Russia and Durmishidze Institute of Biochemistry and Biotechnology, Georgia respectively. From 2006 to date he is the Head of Laboratory of Plant Biopolymers at the Tbilisi State Medical University Institute of Pharmacochemistry. He has been a Visiting Scientist at Utrecht University (Faculty of Pharmacy), The Netherlands by University Scholarship and The Netherlands Organization for Scientific Research (NWO) Scholarship Scientific Program. He has published more than 66 papers in reputed journals.
The structure elucidation of the main structural element of high molecular fractions from medicinal plants of Symphytum asperum, S. caucasicum, S. officinale and Anchusa italica (Boraginaceae) was carried out. According to 13C, 1H NMR and 2D heteronuclear 1H/13C HSQC spectral data of these preparations was found that the main component of these preparations is regularly substituted polyoxyethylene namely poly [3-(3, 4-dihydroxyphenyl) glyceric acid] (PDPGA) or poly [oxy-1-carboxy-2-(3, 4-dihydroxyphenyl) ethylene]. PDPGA represents a new class of natural polyethers with a residue of 3-(3, 4-dihydroxyphenyl) glyceric acid as the repeating unit. Most of the carboxylic groups of PDPGA from A. italica unlike the polymer of Symphytum species according to 2D DOSY experiment are methylated. Such caffeic acid-derived biopolymer to our knowledge has not been known and has been identified for the first time. Besides, the racemic and enantio-selective synthesis of basic monomeric moiety of PDPGA was carried out. 2, 3-Dihydroxy-3-(3, 4-dihydroxyphenyl) propionic acid (DDPPA) and the virtually pure enantiomers, (+)-(2R,3S)-2, 3-dihydroxy-3-(3, 4-dihydroxyphenyl) propionic acid and (-)-(2S,3R)-2, 3-dihydroxy-3-(3, 4-dihydroxyphenyl) propionic acid were synthesized for the first time via sharpless asymmetric dihydroxylation (AD) of trans-caffeic acid derivatives using the enantiocomplementary catalysts, cinchona alkaloid derivatives (DHQD)2-PHAL and (DHQ)2-PHAL. PDPGA and its synthetic monomer exerted anti-cancer efficacy in vitro and in vivo against androgen-dependent and -independent human prostate cancer (PCA) cells via targeting androgen receptor, cell cycle arrest and apoptosis without any toxicity together with a strong decrease in PSA level in plasma. Overall, this study identifies PDPGA as a potent agent against PCA without any toxicity and supports its clinical application.
Birla Institute of Technology, India
Keynote: Enrichment of essential oil components in Citronella (Cymbopogon winterianus Jowitt): A novel approach
Time : ggrg
Dev Mani Pandey is working as Associate Professor at the Department of Bio-Engineering, Birla Institute of Technology, Ranchi, India. He completed his PhD in Plant Physiology from Chaudhary Charan Singh, Haryana Agriculture University, Hisar, India. He was Post-doctoral fellow in South Korea and worked in the areas of plant molecular biology and functional genomics. He was also invited as Consultant to Plant Breeding, Genetics and Biotechnology, IRRI, Los Banos, Philippines. He has presented his research findings and chaired the Session at national and international symposium/conferences/congress in India and abroad as well as organized international symposium successfully. He has published several research papers in peer reviewed journals. He is the principal investigator of Sponsored project as well as guide of many PhD Scholars. His research areas and scientific expertise includes molecular biology, functional genomics, stress physiology and bioinformatics approaches on crop plants using recent biotechnological tools
Citronella oil is one of the essential oils obtained from the leaves and stems of Cymbopogon winterianus and is extensively used as a mosquito repellent, in perfume industry, shows antibacterial and insecticidal activity; effective inhibitor of biodegrading and storage contaminating fungi and in fruit juice preservation, repelling properties on the tick and free radical scavenging. This oil contains medicinally important compounds like nerol, citronellol and geraniol, etc. that are synthesized during its metabolic pathways. Since geraniol dehydrogenase is responsible for the degradation of nerol, citronellol and geraniol. Characterisation of these compounds was performed using HPTLC, ESI MS and FTIR techniques. Therefore, looking the strategies to inactivate geraniol dehydrogenase enzyme by blocking gene encoding this enzyme might be helpful to enrich the content of above mentioned compounds. Also, in silico analysis of GDH gene via molecular docking with potent inhibitors and subsequent study on their individual interactions in enzyme- inhibitor assays will be preliminary yet beneficial for blocking the enzyme activity. The effect of selected inhibitors on the suspension cultures derived from callus proved to be a novel approach for pharmaceutical and cosmetic industry thereby accumulating the essential oil components in vivo.